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Repeat the rinsing with PBS three times and then add 300 L of FITC-phalloidin (Solarbio, Beijing, China) solution, incubate for 30 min in the dark, then wash three times with PBS, Add DAPI (4,6-diamidino-2-phenylindole) staining solution (Solarbio, Beijing, China) for 10 min and seal with glycerol