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MTT assay mainly reflects the damage to cellular mitochondria, and it is commonly used for in vitro cytotoxicity (including skin irritation) measurement

But if the stomach cant absorb B12 appropriately, swallowing more capsules is often not enough

Notably, GPER has sex-specific effects: in female animal models, it markedly increases insulin sensitivity, whereas in males, it primarily curbs weight gain and increases glucose tolerance [79]