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The elution buffer typically contains 10-50 mM reduced glutathione in a suitable buffer (e.g., TBS)

Moreover, HGF downregulates the expression of thrombospondin 1, which negatively regulates angiogenesis (Zhang et al., 2003

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The fractions were lysed with the modified NETN buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 0.5% Nonidet P-40, 100 U/mL MNase, 100 U/ml Benzonase) plus protease inhibitor at 37 C for 20 min