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Following this, JC-1 dye (Chem Cruz, SC 364116 A) (1 g/ml in DMSO) was added to them and incubated in the CO 2 incubator for 30 min

Ethics declarations Conflict of Interest Diego Maria Michele Fornasari received fees as congress speaker or consultant from the following pharmaceutical companies: Angelini, Abiogen, Alfasigma (the manufacturer of acetyl-l-carnitine), Chiesi, Istituto Gentili, Grunentahl, Molteni, Sandoz, SPA, Viatris, Zambon