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Cells of 2 L culture were resuspended in buffer 1 (100 mM HEPES-NaOH pH 7.5, 10 mM MgCl 2 ,150 mM NaCl) or in buffer 1 containing 50 mM NaCl, supplemented with 2.5 units/mL Turbo Nuclease (Jena Bioscience) and disrupted by a single passage through a french press at 14,500 psi