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RNA isolation and quantitative polymerase chain reaction (qPCR) Total RNA was extracted from HUVECs, and real-time quantitative PCR (qPCR) procedures were conducted following established protocols as previously described in refs

2011 (tendon outgrowth and migration, J Appl Physiol)

As such, it is possible that GSH-dependent processes may be more influential on central and peripheral mechanisms driving expiratory chest-muscles than inspiratory chest-muscles