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Thus, unlike previous in vitro biochemical studies or metabolic engineering efforts, which had excluded arGSTs, we set out to confirm our hypothesis by performing coupled enzymatic reactions with 3,4- cis -leucocyanidin ( 6 ) combining clarified Escherichia coli cell lysates expressing At LDOX and the well-studied arGST Ph AN9 from petunia

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The resulting lysate was applied to spin columns, washed sequentially with GW1 and GW2 buffers, and eluted with GE buffer to obtain purified genomic DNA