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Droplet volume was maintained during freeze-thaw cycles using a constant flow of humidified nitrogen generated by combining humid nitrogen (0.010.05 L min 1 ) from a bubbler containing ultrapure water with dry nitrogen gas (0.6 L min 1 ) and passed through a mixing chamber before entering the cooling stage

This metabolic flexibility enables macrophages to adapt to diverse environmental cues, including the presence of therapeutic agents, thereby impacting drug efficacy and resistance (30, 31)

Critically short telomeres signal the cell to enter senescence or apoptosis, contributing to tissue aging and dysfunction

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