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The fluorescein-conjugated goat anti-mouse IgG (115-095-166) and rhodamine-conjugated goat anti-rabbit IgG (111-025-144) antibodies were purchased from Jackson ImmunoResearch Laboratories

Classic symptoms include: Sudden, intense pain in the right upper abdomen or center of the abdomen, often radiating to the right shoulder or back Pain episodes (biliary colic) lasting 30 minutes to several hours, typically occurring after meals Nausea and vomiting accompanying abdominal pain Fever and chills, which may indicate cholecystitis or infection Jaundice (yellowing of skin or eyes), suggesting bile duct obstruction Clay-colored stools or dark urine Persistent indigestion or bloating It is important to note that many gallstones remain asymptomatic and are discovered incidentally on imaging performed for other reasons

During sleep, free radicals are removed as the body repairs and rejuvenates