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At the second timepoint, all participants (control and migraine) completed standardized headache questionnaires inclusive of questions previously validated against ICHD criteria 16 , and a headache neurologist reviewed the data, classifying participants as either meeting criteria for migraine with or without aura based on the ICHD-3 criteria, or not meeting any criteria for migraine

doi: 10.1080/17425255.2016.1206887

No improvement on the other hand would suggest that MTHFR may not be the dominant cause of fatigue even if MTHFR testing is positive