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Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen 0.5 raM L-glutamine, and 5mM cytosine-D-arabinofuranoside from Sigma added at 2 days in vitro

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GHK-Cu is a biologically active peptide found naturally in the body

BPC 157 (L-Valine L-Glutamine L-Glycine L-Proline L-Lysine L-Alanine L-Arginine L-Histidine L-Leucine L-Tyrosine L-Serine L-Threonine L-Isoleucine L-Trypophan L-Methionine)BPC-159 (L-Valine L-Glutamine L-Glycine L-Proline L-Lysine L-Alanine L-Arginine L-Histidine L-Leucine L-Tyrosine L-Serine L-Threonine L-Isoleucine L-Tryptophan L-Methionine)