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trial of 45 men using a GHK peptide complex with 5-aminolevulinic acid for 6 months reported 52 to 72 additional hairs per measurement area, with A 2020 double-blind, placebo-controlled study in 60 men with androgenetic alopecia reported increases in hair count and density over 6 months of twice-daily topical application

The following primary antibodies were listed: -SMA (1:2000, Abcam Cat# ab7817), LRP1 (1:2000, Abcam Cat# ab92544), LRP1 (1:2000, Sigma-Aldrich Cat# L2295), COL1a1(1:2000, Cell Signaling Technology Cat# 72026), ECM1 (1:2000, Sigma-Aldrich Cat# HPA027241), Flag M2 (1:2000, Sigma-Aldrich Cat# F3165), HA-Tag (1:2000, Cell Signaling Technology Cat# 3724), Fibronectin (FN) (1:2000, Cell Signaling Technology Cat# 26836), GST(1:2000, Cell Signaling Technology Cat# 2622), Strep-tag II(1:2000, Abcam Cat# ab184224), AKT(1:2000, Cell Signaling Technology Cat# 4691), p-AKT(1:2000, Cell Signaling Technology Cat# 4060), mTOR(1:2000, Cell Signaling Technology Cat# 2983), p-mTOR(1:2000, Cell Signaling Technology Cat# 5536), FoxO1(1:2000, Cell Signaling Technology Cat# 2880), -Actin (1:5000, Cell Signaling Technology Cat# 4967) and -Tubulin(1:5000, Cell Signaling Technology Cat# 2144)

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