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The column was washed with acetonitrile for 10 min followed by a linear gradient back to the initial mixture of 30% acetonitrile with the flow rate 1 ml/min

2DF, dose-dependent treatment with SP reduced the proportions of M1-like macrophages as well as increased the proportions of anti-inflammatory M2-like macrophages (mannose receptor CD206 as the phenotypic marker of M2-like macrophages), suggesting that SP inhibited the polarization of proinflammatory M1-like macrophages and promoted the differentiation of anti-inflammatory M2-like macrophages

BMC Med (2023) 21:179