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As SPHKAP has previously been shown to bind to the mitochondrial cristae junction regulator and MICOS complex component MIC19 40 , a prominent PKA substrate involved in both cristae assembly during mitochondrial remodelling 43 and in regulation of ERMCSs, we next tested whether exendin-4 stimulation would trigger MIC19 phosphorylation in WT INS-1 832/3 cells using a MIC19 immunoprecipitation strategy coupled with western blotting with an antibody against PKA phosphosites (RRxpS/T) 44

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This peptide, which he named exendin-4 , was approximately 53% identical in amino acid sequence to human GLP-1, but with one critical difference: while human GLP-1 is degraded by an enzyme called DPP-4 within minutes of being released, exendin-4 was resistant to that breakdown

J Pharmacol Exp Ther 369(1):129141