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Blood was collected into tubes containing 1.25 mg EDTA and aprotinin (500 kallikrein-inhibiting units/ml blood) (Wako Pure Chemical Industries), and all samples were centrifuged within 2 h of collection

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Increased specific interfacial areas, more controllable interfacial interactions, higher overall compliance, and achievable loads are among the advantages of nanofiller technology over conventional fillers like carbon fibers, glass fibers, metal particles, wood sheets, and inorganic mineral particles [38]