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Two robust regression LME models (package(nlme)(Pinheiro et al., 2023)) were estimated for each brain region to investigate the relationship between metabolite (GSH or GABA+) levels and diagnosis group, age, and sex, accounting for the random intercept of each participant

These effects are tentatively mediated via arachidonic acid and the increased expression of the dopamine transporter within the lateral septum (Reddy et al., 2016)

This approach adds a few minutes of preparation time upfront but protects the integrity of every subsequent experimental run