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Sample lysates were separated via 414% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), following which the proteins were transferred to polyvinylidene difluoride (PVDF) membranes

Specifically, increased expression of peroxisome proliferator-activated receptor- coactivator-1 protein, a key regulator of mitochondrial biosynthesis, disrupts the mitochondrial structure and leads to dysfunctional proliferation, which is involved in the development of certain cardiac diseases, such as heart failure and atherosclerosis (45)

doi: 10.1038/srep00266 87 PruettSBObiriNKielJL

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