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For cell sorting, cell suspensions were stained with FITC-conjugated human Ep-CAM antibody and Alexa Fluor APC-conjugated human C-Kit for 30 minutes on ice

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The microsomes were incubated with 500 M solutions of Dihexa, piroxicam, verapamil, and 7-ethoxycoumarin, as controls for low, moderate, and highly metabolized compounds, respectively, in 0.1 M Tris buffer with an NADPH-regenerating system at 37C