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Both experimental and clinical studies have reported iron accumulation, enhanced lipid peroxidation, and reduced glutathione peroxidase 4 ( GPX4 ) expression in DKD kidney tissue, supporting ferroptosis as a potential mechanistic link between tubular injury, oxidative stress, and renal fibrosis (Wu and Chen, 2022)
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Bilirubin and enzyme activity To determine the serum levels of aspartate transaminase, alanine transaminase (IU/L), and total bilirubin (mol/L), blood samples were collected immediately after euthanasia and were centrifuged for 15 min at 3000 rpm
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