Samples were fixed in 10% buffered formalin overnight at 4C, dehydrated with increasing concentrations of ethanol, embedded in paraffin, cut into 5 m sections, and stained with hematoxylin and eosin (HE) or Massons Trichrome Stain Kit (Sigma-Aldrich)
The level of ROS in the body increases with age and as a result of negative lifestyle factors such as smoking [4] and obesity [5]
doi: 10.3389/fimmu.2024.1473203 Received 30 July 2024 Accepted 09 September 2024 Published 27 September 2024 Volume 15 - 2024 Edited by Bufu Tang, Fudan University, China Reviewed by Hongquan Wang, Peking University Aerospace School of Clinical Medicine, China Jindong Xie, Sun Yat-sen University Cancer Center (SYSUCC), China Dechao Feng, University College London, United Kingdom Updates Copyright 2024 He, Li, Wu, Liu, Li, Zheng and Lai
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