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After five washing steps with PBS-Tween, the secondary antibody (Alexa Fluor 555 anti-mouse, Life Technologies, 1:200) was added in 2% BSA/PBS-Tween together with Hoechst 33342 (1 g/ml) for 2 h

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This variation is due to genotypic plasticity as revealed through the upregulation of antioxidative enzyme activities and the accumulation of antioxidants

Recommended testing schedule Baseline (before starting): Complete metabolic panel, CBC, lipid panel, HbA1c (even if not diabetic), B12, folate, vitamin D, thyroid panel, and fasting insulin