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In skeletally mature female rats, moderate energy restriction over 12 weeks had minimal negative effects on bone, but severe restriction significantly reduced bone mass and levels of IGF-1, leptin, and estradiol

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POMC-derived peptides including adrenocorticotropin (ACTH), alpha melanocyte stimulating hormone (-MSH), -MSH and -endorphins, MC1R, adrenergic and cholinergic agents, and growth factors, cytokines, or nitric oxide (NO) secreted in the local microenvironment are all involved in the regulation of melanogenesis [6]