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After transfer of 100 L of the respective cell suspensions into a black, clear-bottom 96-well plate (Nunc, Rochester, NY), 5 mM of the substances of interest: buffer, reduced (Sigma-Aldrich, CAS-70-18-8, GSH) or oxidized (Sigma-Aldrich, CAS-27025-41-8, GSSG) glutathione, cysteine or cystine, -mercapthoethanol or DTT were added

Always use airtight, light-resistant containers for storage

Gonads were dissected as for germline antibody staining and fixed in 3% paraformaldehyde/0.1M K 2 HPO 4 (pH 7.2) solution for 10-20 min, followed by -20C methanol fixation for 10 min

These unregulated treatments pose real risks to patients, and stronger enforcement is critical